ca074-me (Enzo Biochem)
90
Structured Review
Enzo Biochem
ca074-me
Ca074 Me, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ca074-me/mitotempo/pmc03990545-36-0-4
Average 90 stars, based on 1 article reviews
Ca074 Me, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ca074-me/mitotempo/pmc03990545-36-0-4
Average 90 stars, based on 1 article reviews
ca074-me - by Bioz Stars,
2026-09
90/100 stars
Images
1) Product Images from "Hypoxia Enhances the Antiglioma Cytotoxicity of B10, a Glycosylated Derivative of Betulinic Acid"
Article Title: Hypoxia Enhances the Antiglioma Cytotoxicity of B10, a Glycosylated Derivative of Betulinic Acid
Journal: PLoS ONE
doi: 10.1371/journal.pone.0094921
Figure Legend Snippet: To block lysosomal acidification we used bafilomycin A1 (BafA1). A, LN-308 or LNT-229 cells were co-treated with 10 nM BafA1 and 10 µM B10 under hypoxic conditions (1% O 2 ) for 48 h. Cell density was assessed by CV (n = 4, SD, *p<0.05). B, cell death was quantified by LDH-release after treating LN-308 or LNT-229 cells with BafA1 and B10 under hypoxic conditions (0.1% O 2 ) for 19 h (LN-308) or 27 h (LNT-229) (n = 4, SD, * = p<0.05). To inhibit cathepsin B we used the cathepsin inhibitor CA074-Me. C, LN-308 or LNT-229 cells were co-treated with 10 µM CA074-Me and 10 µM B10 under hypoxic conditions (1% O 2 ) for 48 h. Cell density was assessed by CV (n = 4, SD, *p<0.05). D, cell death was quantified by LDH release after treating LN-308 or LNT-229 cells with CA074-Me and B10 under hypoxic conditions (0.1% O 2 ) for 19 h (LN-308) or 25 h (LNT-229) (n = 4, SD, *p<0.05).
Techniques Used: Blocking Assay
Figure Legend Snippet: Using a pLKO.1-based lentivirus LN-308 cathepsin B (CTSB) knockdown cells and control cells (non-targeting sequence, Scrsh) were generated. CTSB gene suppression was confirmed by qRT-PCR (A) and CTSB activity (B) (n = 3, SD, *p<0.05). The effect of the cathepsin inhibitor CA074-Me (10 µM) on cathepsin B activity in control cells (Scrsh) is shown in B. C, B10-mediated cytotoxicity under 0.1% O 2 in LN-308 control cells and CTSBsh cells was quantified by LDH-release assay (n = 4, SD, *p<0.05).
Techniques Used: Sequencing, Generated, Quantitative RT-PCR, Activity Assay, Lactate Dehydrogenase Assay
Related Articles
Blocking Assay:Article Title: Synergistic apoptotic response between valproic acid and fludarabine in chronic lymphocytic leukaemia (CLL) cells involves the lysosomal protease cathepsin B Article Snippet: Chemical inhibitors of cathepsins, Article Title: Hypoxia Enhances the Antiglioma Cytotoxicity of B10, a Glycosylated Derivative of Betulinic Acid Article Snippet: Article Title: Acid Ceramidase Expression Modulates the Sensitivity of A375 Melanoma Cells to Dacarbazine Article Snippet: Dacarbazine, doxycycline, N -acetylcysteine, pepstatin A, leupeptin, and monodansylcadaverine were supplied from Sigma, blasticidin and hygromycin B were from Invitrogen, Sequencing:Article Title: Synergistic apoptotic response between valproic acid and fludarabine in chronic lymphocytic leukaemia (CLL) cells involves the lysosomal protease cathepsin B Article Snippet: Chemical inhibitors of cathepsins, Article Title: Hypoxia Enhances the Antiglioma Cytotoxicity of B10, a Glycosylated Derivative of Betulinic Acid Article Snippet: Article Title: Acid Ceramidase Expression Modulates the Sensitivity of A375 Melanoma Cells to Dacarbazine Article Snippet: Dacarbazine, doxycycline, N -acetylcysteine, pepstatin A, leupeptin, and monodansylcadaverine were supplied from Sigma, blasticidin and hygromycin B were from Invitrogen, Generated:Article Title: Synergistic apoptotic response between valproic acid and fludarabine in chronic lymphocytic leukaemia (CLL) cells involves the lysosomal protease cathepsin B Article Snippet: Chemical inhibitors of cathepsins, Article Title: Hypoxia Enhances the Antiglioma Cytotoxicity of B10, a Glycosylated Derivative of Betulinic Acid Article Snippet: Article Title: Acid Ceramidase Expression Modulates the Sensitivity of A375 Melanoma Cells to Dacarbazine Article Snippet: Dacarbazine, doxycycline, N -acetylcysteine, pepstatin A, leupeptin, and monodansylcadaverine were supplied from Sigma, blasticidin and hygromycin B were from Invitrogen, Quantitative RT-PCR:Article Title: Synergistic apoptotic response between valproic acid and fludarabine in chronic lymphocytic leukaemia (CLL) cells involves the lysosomal protease cathepsin B Article Snippet: Chemical inhibitors of cathepsins, Article Title: Hypoxia Enhances the Antiglioma Cytotoxicity of B10, a Glycosylated Derivative of Betulinic Acid Article Snippet: Article Title: Acid Ceramidase Expression Modulates the Sensitivity of A375 Melanoma Cells to Dacarbazine Article Snippet: Dacarbazine, doxycycline, N -acetylcysteine, pepstatin A, leupeptin, and monodansylcadaverine were supplied from Sigma, blasticidin and hygromycin B were from Invitrogen, Activity Assay:Article Title: Synergistic apoptotic response between valproic acid and fludarabine in chronic lymphocytic leukaemia (CLL) cells involves the lysosomal protease cathepsin B Article Snippet: Chemical inhibitors of cathepsins, Article Title: Hypoxia Enhances the Antiglioma Cytotoxicity of B10, a Glycosylated Derivative of Betulinic Acid Article Snippet: Article Title: Acid Ceramidase Expression Modulates the Sensitivity of A375 Melanoma Cells to Dacarbazine Article Snippet: Dacarbazine, doxycycline, N -acetylcysteine, pepstatin A, leupeptin, and monodansylcadaverine were supplied from Sigma, blasticidin and hygromycin B were from Invitrogen, Lactate Dehydrogenase Assay:Article Title: Synergistic apoptotic response between valproic acid and fludarabine in chronic lymphocytic leukaemia (CLL) cells involves the lysosomal protease cathepsin B Article Snippet: Chemical inhibitors of cathepsins, Article Title: Hypoxia Enhances the Antiglioma Cytotoxicity of B10, a Glycosylated Derivative of Betulinic Acid Article Snippet: Article Title: Acid Ceramidase Expression Modulates the Sensitivity of A375 Melanoma Cells to Dacarbazine Article Snippet: Dacarbazine, doxycycline, N -acetylcysteine, pepstatin A, leupeptin, and monodansylcadaverine were supplied from Sigma, blasticidin and hygromycin B were from Invitrogen, Staining:Article Title: Synergistic apoptotic response between valproic acid and fludarabine in chronic lymphocytic leukaemia (CLL) cells involves the lysosomal protease cathepsin B Article Snippet: Chemical inhibitors of cathepsins, Article Title: Hypoxia Enhances the Antiglioma Cytotoxicity of B10, a Glycosylated Derivative of Betulinic Acid Article Snippet: Article Title: Acid Ceramidase Expression Modulates the Sensitivity of A375 Melanoma Cells to Dacarbazine Article Snippet: Dacarbazine, doxycycline, N -acetylcysteine, pepstatin A, leupeptin, and monodansylcadaverine were supplied from Sigma, blasticidin and hygromycin B were from Invitrogen, Western Blot:Article Title: Synergistic apoptotic response between valproic acid and fludarabine in chronic lymphocytic leukaemia (CLL) cells involves the lysosomal protease cathepsin B Article Snippet: Chemical inhibitors of cathepsins, Article Title: Hypoxia Enhances the Antiglioma Cytotoxicity of B10, a Glycosylated Derivative of Betulinic Acid Article Snippet: Article Title: Acid Ceramidase Expression Modulates the Sensitivity of A375 Melanoma Cells to Dacarbazine Article Snippet: Dacarbazine, doxycycline, N -acetylcysteine, pepstatin A, leupeptin, and monodansylcadaverine were supplied from Sigma, blasticidin and hygromycin B were from Invitrogen, Purification:Article Title: Synergistic apoptotic response between valproic acid and fludarabine in chronic lymphocytic leukaemia (CLL) cells involves the lysosomal protease cathepsin B Article Snippet: Chemical inhibitors of cathepsins, Article Title: Hypoxia Enhances the Antiglioma Cytotoxicity of B10, a Glycosylated Derivative of Betulinic Acid Article Snippet: Article Title: Acid Ceramidase Expression Modulates the Sensitivity of A375 Melanoma Cells to Dacarbazine Article Snippet: Dacarbazine, doxycycline, N -acetylcysteine, pepstatin A, leupeptin, and monodansylcadaverine were supplied from Sigma, blasticidin and hygromycin B were from Invitrogen, Incubation:Article Title: Synergistic apoptotic response between valproic acid and fludarabine in chronic lymphocytic leukaemia (CLL) cells involves the lysosomal protease cathepsin B Article Snippet: Chemical inhibitors of cathepsins, Article Title: Hypoxia Enhances the Antiglioma Cytotoxicity of B10, a Glycosylated Derivative of Betulinic Acid Article Snippet: Article Title: Acid Ceramidase Expression Modulates the Sensitivity of A375 Melanoma Cells to Dacarbazine Article Snippet: Dacarbazine, doxycycline, N -acetylcysteine, pepstatin A, leupeptin, and monodansylcadaverine were supplied from Sigma, blasticidin and hygromycin B were from Invitrogen, Polyacrylamide Gel Electrophoresis:Article Title: Synergistic apoptotic response between valproic acid and fludarabine in chronic lymphocytic leukaemia (CLL) cells involves the lysosomal protease cathepsin B Article Snippet: Chemical inhibitors of cathepsins, Article Title: Hypoxia Enhances the Antiglioma Cytotoxicity of B10, a Glycosylated Derivative of Betulinic Acid Article Snippet: Article Title: Acid Ceramidase Expression Modulates the Sensitivity of A375 Melanoma Cells to Dacarbazine Article Snippet: Dacarbazine, doxycycline, N -acetylcysteine, pepstatin A, leupeptin, and monodansylcadaverine were supplied from Sigma, blasticidin and hygromycin B were from Invitrogen, |